Serum

Human serum is a widely used matrix in clinical and biological studies.

Biological samples of blood serum for laboratory research are collected in plastic or glass tubes, partially vacuumed. This ensures that the internal air pressure in them is lower than atmospheric.

Serum tubes are gently inverted at room temperature to achieve complete coagulation before performing the same centrifugation process as for plasma. Each serum sample was aliquoted into three fractions of 1.0 ml each and stored at -80°C. Higher concentrations of metabolites in blood serum make it possible to obtain more sensitive results for the determination of biomarkers.